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. 2014 Nov 20;43(3):e17. doi: 10.1093/nar/gku1198

Figure 6.

Figure 6.

Enhancing the CRISPR/Cas9-mediated targeting efficiency by siRNAs against Ku70, Lig4 and XRCC4 (KLX siRNAs). (A) miR-21 KO in HEK293T cells with representative images of colonies. (B) Relative colony number for control siRNA or KLX siRNAs for miR-21 gRNAs. (C) Junction PCR products using primers miR-21-recom-5.2 and EF1-Seq-3.2. (D) UCA1 KO in HCT-116 cells with representative images of colonies. (E) Relative colony number for control siRNA or KLX siRNAs for UCA1-gRNAs. (F) Junction PCR products using primers UCA1-Recom-5.1 and EF1–seq-3.2. Pictures were taken 7 days after puromycin selection for both miR-21 and UCA1 KO. Values in (B) and (E) are means of ± SE (n = 3). *P < 0.05.