Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jan 20;43(3):1407–1417. doi: 10.1093/nar/gku1319

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 6. — (A) Restriction map of the probes used in the DNA bending assay (from vector pBend4); the oblong indicates location of the DNA 6 in the DNA probe. (B) Circular permutation assay to determine deviation from linearity of DNA on binding to HlyU_Vc. (C) Plot of μ_bound/μ_free (‘r’, the ratio of mobility (μ) of the bound form and the free probe for each restriction enzyme digested fragment with the DNA6 insert) versus the position (d, in bp) of the DNA insert from the 5′ end of each restriction fragments. The minimum and maximum values of the ratio were used to calculate α (bend angle) according to Equation (1). (D) DNA-HlyU_Vc model after 10 ns MD simulation.