Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jan 23;43(3):1795–1803. doi: 10.1093/nar/gku1330

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 2. — Ref truncations have distinct affinities for ssDNA and dsDNA in comparison to WT Ref. Equilibrium binding isotherms of WT Ref and truncations in presence of labeled ssDNA or dsDNA as monitored by fluorescence polarization. All data are the average of at least three experiments. Error bars are one standard deviation from the mean. (A) ϕW39 ΔN21 Ref and ϕW39 ΔN47 Ref show reduced affinity for ssDNA in comparison to WT Ref. (B) ϕW39 ΔN21 Ref and ϕW39 ΔN47 Ref also show a decreasing affinity for dsDNA as more of the N-terminus is removed in comparison to WT Ref. (C) Table of apparent K_d,app values for each Ref protein analyzed from at least three replicates. No DNA binding was observed with the ϕW39 ΔN74 Ref or P1 ΔN76 Ref.