Figure 2.

LYS20 interacted genetically with DNA damage response genes. DNA damage sensitive strains were transformed with vector or with the lys20-cat mutant (lys20-E155A) that was competent for moonlighting activity but not for lysine biosynthesis. Increased dosage of lys20-cat improved the DNA damage sensitivity of esa1–414 and tel1Δ, but interfered in rad6Δ cells. The lys20-cat mutant was used because it was more proficient in suppressing esa1–414 compared to wild-type LYS20 (Figure 1E).