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. 2015 Jan 13;43(3):1659–1670. doi: 10.1093/nar/gku1406

Figure 2.

Figure 2.

53BP1 promotes DNA DSB repair and genomic stability in cells with deficient DNA-PKcs. (A) Relative DNA-PKcs mRNA levels from H1299 cells transfected with shRNA against DNA-PKcs (DNA-PKcssh) or a control (consh). (B) Percentage of cells with ≥10 γ-H2AX foci with or without 53BP1 depletion in irradiated G1-enriched H1299 cells with additional DNA-PKcs depletion. (C) Western blot showing 53BP1 depletion in M059J cells. (D) Percentage of cells with γ-H2AX foci in irradiated asychronous M059J cells. (E) Olive moment in the Comet assay with or without 53BP1 depletion in G1-enriched M059J cells. P-values were calculated with the Student's t-test. Error bars represent the SD of three independent experiments. (F) 53BP1 depletion increases the frequency of both Chme and Chid breaks in M059J cells harvested at 15 h after IR (upper panel). The methods are the same as described in Figure 1E. (G) A representative spectrum of individual repair products recovered from G1-enriched H1299 cells with or without 53BP1 transfected with EcoRV-linearized pGL3-MCS substrate (59) (upper panel). The frequency of precise NHEJ was determined by DNA sequencing (bottom panel). P-values were calculated by Chi-square test.