Figure 1.

Frequency of IL-17A- and IFN-γ-producing cells among memory T cells from female aged Balb/c mice. (A) CD4⁺ enriched cells from lymph nodes and spleen of aged Balb/c mice were stimulated with PMA/ionomycin, labeled with the IL-17A- and IFN-γ-cytokine secretion kit, analyzed and sorted by flow cytometry. Rectangles in the plots indicate the sorting gates. Frequencies of IFN-γ⁻IL-17A⁺ Th17 cells, IFN-γ⁺IL-17A⁻ Th1 cells, IFN-γ⁻IL-17A⁻ Th-mem cells and IFN-γ⁻IL-17A⁻ naive T (Tnaive) cells are depicted. (B) Analysis of sorted naive T cells, Th-mem, Th1 and Th17 cells. Numbers indicate the frequency of cells within quadrants. Representative dot plots of 23 independently performed sorts are shown.