Table 1. Replication profiles of mutants in this study with and without UV treatment.
| Cell | UV | ΣIdU/ΣDNA (%) | Fork density (per Mb) | Average inter origins distances (kb) | Fork speed (kb/min) | Fiber length (kb) | Mb of DNA analyzed/number of fiber |
|---|---|---|---|---|---|---|---|
| Wild-type | − | 47.05 | 8.63 | 81 ± 7 | 1.36 ± 0.05 | 368 ± 25 | 36.7/111 |
| + | 44.21 | 8.00 | 76 ± 5 | 1.27 ± 0.06 | 360 ± 24 | 24.6/75 | |
| pold3 | − | 46.58 | 10.42 | 81 ± 4 | 1.28 ± 0.03 | 345 ± 17 | 62.4/190 |
| + | 31.03 | 7.35 | 88 ± 7 | 1.14 ± 0.04 | 359 ± 23 | 28.8/89 | |
| polζ/polη | − | 41.53 | 8.54 | 78 ± 5 | 1.40 ± 0.04 | 312 ± 14 | 42.9/153 |
| + | 41.96 | 8.51 | 82 ± 4 | 1.24 ± 0.03 | 367 ± 28 | 48.2/149 | |
| polζ/polη | − | 43.98 | 8.63 | 91 ± 8 | 1.40 ± 0.06 | 383 ± 24 | 21.9/58 |
| pold3/tet-ON | + | 46.18 | 8 | 78 ± 7 | 1.41 ± 0.08 | 326 ± 28 | 15.1/49 |
| polζ/polη | − | 49.57 | 6.94 | 85 ± 6 | 1.43 ± 0.06 | 341 ± 25 | 31.7/104 |
| pold3/tet-OFF | + | 53.27 | 8.32 | 73 ± 5 | 1.13 ± 0.05 | 304 ± 21 | 26.8/97 |
Profile of the DNA replication kinetics in indicated cells with or without UV irradiation was analysed by molecular combing.