Figure 6.

Combinatorial treatment of LY294002 and SAHA overcomes Oct4-mediated drug resistance in vivo. (A) Nude mice bearing xenografts of A549 stable clone, vector (left) or Oct4#1 (right), were treated with DMSO, LY294002 (25 mg/kg) and/or SAHA (30 mg/kg). Tumor volume and image of xenografts are shown. (B) Tumor weight of vector and Oct4#1 xenografts. (C) IHC analyses of Oct4 and PTEN proteins. (D) IHC analysis of cleaved Caspase-3 proteins. For all IHC images, the insets are a higher magnification of the boxed areas. Original magnification × 200. For all graphs, data are mean ± SEM. (n = 5 mice per group). P-values determined using two-tailed Student's t-test. *P<0.05; **P<0.01; ***P<0.001.