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. 2015 Jan 21;43(3):1593–1608. doi: 10.1093/nar/gkv024

Figure 7.

Figure 7.

TNC expression is required for Oct4-mediated cancer invasiveness in vitro and in vivo. (A and B) Transwell invasion/migration assay was performed in A549 stable clones (Oct4#1 and Oct4#2) after TNC knockdown (si-TNC) or control siRNA (si-Ctrl). Representative image (A) and quantitation (B) are shown. Data are mean ± SEM. (n = 3). (C) Western blot analysis of TNC and Oct4 protein levels in cells from (A and B). (D and E) Transwell invasion/migration assay was performed in si-Ctrl, TNC, si-Oct4, si-Oct4/TNC cells. Representative image (D) and quantitation (E) are shown. Data are mean ± SEM. (n = 3). (F) Western blot analysis of TNC and Oct4 protein levels in cells from (D and E). (G) Representative lung images of mice intravenously injected via tail vein with si-Ctrl, TNC, si-Oct4, si-Oct4/TNC A549 cells (left). Tumor nodules were indicated by arrows. Number of tumor nodules in lungs per mice is shown (right). Data are mean ± SEM. (n = 8 mice per group). P-values determined using two-tailed Student's t-test. *P<0.05; **P<0.01; ***P<0.001. Original magnification × 100 for all microscopic images.