Skip to main content
. 2015 Jan 21;43(3):1609–1625. doi: 10.1093/nar/gkv026

Figure 1.

Figure 1.

Sp1 activates transcriptional initiation/activation of RelA/p65 via a proximal promoter GC-rich element (bp, −30 to −1). (A, B) RT-qPCR and western blot analysis of RelA/p65 expression. Knockdown of Sp1 mRNA resulted in a potent decrease in RelA/p65 expression at both the mRNA and protein levels. *P < 0.05. (C) Transient transfection and transcription assays, and identification of a cis-regulatory element critical in transcriptional initiation/activation of RelA/p65 by Sp1 in HEK293 cells. The structures of various pGL2-RelA/p65-Luc constructs are shown on the left. +1 (Tsp), transcription start point. Potential Sp1-binding GC-boxes are indicated (open circles, as analyzed by MacVector program). The data presented are the average of three independent assays. Luciferase activity was normalized to total protein concentration. Bars indicate standard deviation. *P < 0.05, statistically significant.