Table 2. Guinier and Porod analysis of peak fractions from PPR10:atpH and MBP-PPR10:atpH SEC-SY-SAXS.

Frame no.	I(0)	Radius of gyration, Rg (Å)	Guinier range (q)	Maximum dimension, Dmax (Å)	P(r) Range (q)	Porod invariant, Vp (Å3)	Molecular mass, Mr, from Vp (Da)	Oligomeric state from Mr
552–570	0.007 ± 0.000	37.394 ± 2.299	0.014–0.034	130.1	0.014–0.25	165,328	97,251	1.11
570–590	0.006 ± 0.000	36.055 ± 3.811	0.014–0.034	126.2	0.014–0.25	162,893	95,819	1.09
590–610	0.005 ± 0.000	36.456 ± 3.967	0.014–0.034	127.6	0.014–0.25	165,752	97,501	1.11
610–630	0.004 ± 0.000	36.773 ± 5.734	0.014–0.034	126.4	0.014–0.25	158,006	92,944	1.06
630–650	0.003 ± 0.000	35.034 ± 9.020	0.014–0.034	123.9	0.014–0.25	187,843	110,495	1.26
650–670	0.002 ± 0.000	38.532 ± 5.436	0.014–0.034	130.0	0.014–0.25	162,642	95,671	1.09
215–220	0.012 ± 0.000	67.459 ± 6.188	0.012–0.025	250.0	0.012–0.35	210,905	124,061	0.94
221–226	0.012 ± 0.000	61.199 ± 1.868	0.012–0.025	250.0	0.012–0.35	203,698	135,607	1.03
227–233	0.007 ± 0.000	55.053 ± 1.385	0.012–0.025	250.0	0.012–0.35	204,390	143,800	1.09
234–239	0.005 ± 0.000	54.975 ± 2.248	0.012–0.025	250.0	0.012–0.35	184,072	129,100	0.98
240–245	0.003 ± 0.000	55.947 ± 3.515	0.012–0.025	250.0	0.012–0.35	160,763	112,200	0.85

Forward scatter,I(0), radius of gyration, R_g determined using PRIMUS. Maximum dimension, D_max, and Porod volume,V_p, and molecular mass, M_r, determined using GNOM. Oligomeric state (n) determined from the ratio of M_r(V_p) to M_r (theoretical).