Figure 1.

UV sensitivity and global genomic nucleotide excision repair (GG-NER) activity of transformed human fibroblast cell lines ectopically expressing wild-type or mutant DDB2 protein. (A) Immunoblot analyses of parental WI38 VA13 cells and transformed cell lines ectopically expressing various DDB2 proteins. RAD23B was used as a loading control. Note that reactivity of the anti-DDB2 antibody is slightly compromised by mutations in the N-terminal tail. (B) Clonogenic UV survival assay of the established cell lines. (C and D) Repair kinetics of UV-induced 6-4PPs (C) and CPDs (D). The XPC-deficient cell line (XP4PASV) was used as a negative control. For (B), (C) and (D), mean values and standard errors were calculated from three independent experiments.