Fig. 1.

Flotillin-1 is essential for poly-I:C-induced inflammatory gene expression. a and b Western blot and densitometry for the proteins indicated in HUVEC treated with or without poly-I:C, lipopolysaccharide (LPS) and lipoteichonic acid (LTA, each 10 µg/ml, 3 h) after lentiviral transduction with shRNAs for flotillin-1 (shFlot-1A and B) or GFP (shGFP) (c) qRT-PCR for VCAM-1 relative to RNA polymerase II by 2^-ΔΔCT in HUVEC stimulated with (each 10 µg/ml, 90 min) or without LPS and poly-I:C after transduction with shRNAs for flotillin-1 (shFlot-1A and B), GFP (shGFP) or a scrambled shRNA (shScr). N = 3; *p < 0.05 with vs. without poly-I.C, ^# p < 0.05 shGFP vs. shFlot