Fig. 2.

Flotillin-1 is involved in poly-I:C-induced IRF-3 signaling. a qRT-PCR for the IRF-3-inducible genes IFNβ and CXCL10 in HUVEC stimulated with or without poly-I:C and LPS (each 10 µg/ml, 90 min) after transduction with shRNAs for flotillin-1 (shFlot-1A and B), GFP (shGFP) or a scrambled shRNA (shScr). b and c Western blot analysis for the proteins indicated of nuclear extracts from HUVEC stimulated with poly-I:C (b, 10 µg/ml) and LPS (c, 10 µg/ml) for the times indicated after transduction with shRNA for flotillin-1 (shFlot-1) or GFP (shGFP). pIRF-3 indicated anti-phospho-IRF3 (phospho-Ser 385). Topo-1: DNA-topoisomerase. N = 4; *p < 0.05