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. 2014 Sep 10;109(6):439. doi: 10.1007/s00395-014-0439-4

Fig. 5.

Fig. 5

Flotillin-1 maintains caveolin-1 protein which is required for the poly-I:C response. a Subset of SILAC-mass spectrometric analyses on the effect of shFlot-1 on protein abundance in the detergent-resistant membrane fraction of HUVECs. b Exemplary Western blot and densitometry for the proteins indicated of HUVECs transduced with shGFP or shFlot-1 or transfects with scrambled siRNA (siScr) or caveolin-1 siRNA (siCav-1) with and without stimulation with poly-I:C (10 µg/ml, 180 min), *p < 0.05, n = 4. c Proximity ligation assays for flotillin-1 and caveolin-1 interaction (red) in HUVECs transduced with shGFP or shFlotillin-1. Down total internal reflection fluorescence microscopy (TIRF) image, up epifluorescence. Cells were counterstained with paxillin (green) and DAPI (blue). d and e Exemplary Western blot and densitometry for the proteins indicated of HUVECs transduced with shGFP or shFlot-1 with and without stimulation of thapsigargin 3.3 µmol/l. N = 4 *p < 0.05