Fig. 2.

Eupatilin suppresses mRNAs encoding inflammatory cytokines. (A) CCK assay of eupatilin cytotoxicity. FLS were seeded in 96 well plates, incubated with eupatilin for 24 hr, and incubated with CCK-8 solution for 2 hr. All values are expressed as mean±SEM. CTL (control), treatment with dimethyl sulfoxide. ^*P < 0.01 compared with CTL. (B) FLS were incubated with eupatilin for 24 hr and TNF-α (10 ng/mL) was added for 15 min. The amounts of IL-6 and IL-1β mRNAs were assayed by RT-PCR.