Fig. 1.

Experimental strategy of mTOR signaling in bladder cancer progression in vivo. Bioluminescence imaging confirmed Ku-7-luc cells intravesical instilled mice which were confirmed by bioluminescence imaging were randomised into control, pS6K siRNA, elF4E siRNA, dual pS6K and elF4E, and rapamycin groups. Beginning at 7th days, mice were delivered pS6K siRNA, elF4E siRNA, dual pS6K and elF4E, and rapamycin through a catheter into the bladder lumen, respectively twice a week, and we monitored bladder cancer progression by serial bioluminescence imaging. At 21th days mice were sacrificed for analysis of bladder histophatology.