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. 2015 Feb 17;5:8521. doi: 10.1038/srep08521

Figure 3. Application of IL-36Ra modulates CCL20 expression in late murine APAP-induced liver injury.

Figure 3

(abc) Mice received APAP (n = 11) or APAP/IL-36Ra (n = 12) and were maintained for 48 h. Thereafter, hepatic CCL20 mRNA (a) and protein (b) was determined by realtime PCR and ELISA, respectively. (a) Target mRNA was normalized to that of GAPDH and is shown as percent of APAP alone (means ± SEM). (b) Liver tissue CCL20 content was determined by ELISA, is depicted as pg/500 μg total protein, and shown as means ± SEM. (c) Hepatic CCL2 and CXCL2 mRNA was determined by realtime PCR. Target mRNA was normalized to that of GAPDH and is shown as percent of APAP alone set as 100% (means ± SEM). Statistical analysis, Student's t-test; n.s., not significant.