Figure 5.

msca1-ref Mutants Have Significantly Reduced SAM Size.

(A) and (B) Representative images of cleared SAMs from 14-d-old wild-type (A) and msca1-ref (B) segregating siblings. Dashed arrows indicate reference points for SAM diameter measurements. Bars = 100 μm.

(C) Quantification of SAM size from 14-d-old wild-type (n = 29) and msca1-ref (n = 21) seedlings. Values are mean ± sd. Difference is significant, **P < 0.001.

(D) Neighbor-joining sequence similarity analysis of MSCA1/ABPH2 and its close homologs from Arabidopsis (ROXY1 and ROXY2 are AT3g02000 and AT5g14070, respectively; At1G28480 and At1G03850 are ROXY19 and 18, respectively; Li et al., 2011), sorghum, and rice (MIL1 is OS07G05630). The branches indicate the bootstrap values for 1000 replicates.

(E) Longitudinal section of a 16-d-old shoot apex hybridized by an antisense probe against GRMZM2G480903 3′UTR (350 bp). Bar = 200 μm.