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. 2015 Jan 27;27(1):202–213. doi: 10.1105/tpc.114.133868

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© 2015 American Society of Plant Biologists. All rights reserved.

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Figure 3. — The Constitutive Interaction of UVR8^W285A with COP1 Depends on the C-Terminal VP Pair.

(A) Immunoblot analysis of UVR8 protein expression levels in uvr8-7 and several independent uvr8-7/Pro35S:UVR8^W285,VP-AA (W285A^VP-AA) and uvr8-7/Pro35S:UVR8^W285A (W285A) transgenic lines.

(B) Hypocotyl growth in weak white light. Images of representative individuals (top panel) and quantification of hypocotyl lengths (bottom panel) of 4-d-old seedlings grown under weak white light are shown. Means and se are shown (n > 20).

(C) Coimmunoprecipitation of COP1 in whole cell extracts from seedlings grown for 7 d in white light. IB, immunoblotting; IP, immunoprecipitation.

(D) Quantitative yeast two-hybrid assays were performed in the presence or absence of UV-B. Means and se from three biological replicates are shown. AD, activation domain construct; BD, binding domain construct; EV, empty vector; β-Gal., β-galactosidase; MU, Miller units.