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. 2015 Jan 27;27(1):202–213. doi: 10.1105/tpc.114.133868

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Figure 4. — Induced Expression of UVR8^C44 Leads to HY5 Transcript Accumulation in Arabidopsis Seedlings in the Absence of UV-B.

(A) Immunoblot analysis of YFP protein accumulation induced by β-estradiol in Arabidopsis seedlings. uvr8-7/XVE:YFP alone (YFP) or uvr8-7/XVE:UVR8^C44-UVR8^C44-NLS-UVR8^C44-2A-YFP (3XC44-2A-YFP) was treated for 5 h with 50 μM 17-β-estradiol in DMSO (Estr.) or with DMSO only. Extracts were probed with anti-YFP (top panel) or anti-actin (bottom panel) antibody. Untreated controls (−) were included. 2A encodes a cotranslational cleavage site.

(B) Quantitative real-time PCR analysis of HY5 mRNA accumulation in Arabidopsis seedlings. Seedlings were either treated for 5 h with 50 μM 17-β-estradiol in DMSO or with DMSO only or were left untreated (−) Data were normalized against untreated samples without treatment at time 0. Means and se are shown (n = 3).