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. 2015 Jan 27;27(1):202–213. doi: 10.1105/tpc.114.133868

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Figure 5. — Seedlings Stably Expressing UVR8^C44 Show a Constitutive Photomorphogenic Phenotype.

(A) UVR8 protein levels in uvr8-7, wild-type (Wassilewskija [Ws]), YFP-NLS-C44-expression line 1, 13, and 15, and YFP-YFP-expression line (far right) seedlings.

(B) and (C) Hypocotyl growth in weak white light. Images of representative individuals (B) and quantification of hypocotyl lengths (C) of 4-d-old seedlings grown under white light are shown. Means and se are shown (n > 20).

(D) Transcript levels of CHS in uvr8-7/Pro35S:YFP-NLS-UVR8^C44 transgenic seedlings (lines 1, 13, and 15) compared with a uvr8-7/Pro35S:YFP-YFP line (YFP-YFP) as measured by quantitative RT-PCR analysis. Error bars represent se in triplicate biological measurements.

(E) Coimmunoprecipitation of COP1 in protein extracts from uvr8-7, uvr8-7/Pro35S:YFP-YFP, and uvr8-7/Pro35S:YFP-NLS-UVR8^C44 transgenic seedlings grown for 7 d in white light. IB, immunoblotting; IP, immunoprecipitation.