Figure 6.

UVR8 Interacts with RUP1 and RUP2 Only via Its C27 Domain.

(A) and (B) Yeast two-hybrid growth assay on selective SD/-Trp/-Leu/-His medium in the presence (+) or absence (−) of UV-B light. AD, activation domain construct; BD, binding domain construct; EV, empty vector.

(C) and (D) Quantitative yeast two-hybrid assays were performed in the presence or absence of UV-B light. Means and se from three biological replicates are shown. β-Gal., β-galactosidase; MU, Miller units.

(E) Dimerization of UVR8 in 7-d-old wild-type (Wassilewskija [Ws]) and uvr8-7/Pro35S:UVR8^VP-AA line 11 seedlings. Seedlings were either left unirradiated (−UV) or irradiated with broad-band UV-B light for 15 min (+UV) to induce UVR8 monomerization before being subjected to a recovery period in white light for 0.5 to 4 h. Asterisks indicate nonspecific cross-reacting bands.