Table 1. Mass Composition and Fatty Acid Flux Sharing between the Two Glycerolipid Pathways in Arabidopsis Grown at 10, 22, and 30°C.
Mass of Fatty Acid (mol/1000 mol)a |
sn-2 Position (%)b |
Fatty Acid Entering Prokaryotic Pathway (mol/1000 mol Fatty Acid) | Fatty Acid Entering Eukaryotic Pathway (mol/1000 mol Fatty Acid) | ||
---|---|---|---|---|---|
C16 | C18 | ||||
MGDG | |||||
10°C | 411 | 79 | 21 | 325 | 86 |
22°C | 380 | 76 | 24 | 289 | 91 |
30°C | 387 | 68 | 32 | 263 | 124 |
DGDG | |||||
10°C | 159 | 13 | 87 | 21 | 138 |
22°C | 160 | 16 | 84 | 26 | 134 |
30°C | 179 | 18 | 82 | 32 | 147 |
PC | |||||
10°C | 166 | 2 | 98 | 3 | 163 |
22°C | 176 | 8 | 92 | 14 | 162 |
30°C | 161 | 4 | 96 | 6 | 155 |
PG | |||||
10°C | 101 | 90 | 10 | 91 | 10 |
22°C | 101 | 93 | 7 | 94 | 7 |
30°C | 103 | 95 | 5 | 98 | 5 |
PE | |||||
10°C | 114 | 0 | 100 | 0 | 114 |
22°C | 124 | 0 | 100 | 0 | 124 |
30°C | 106 | 0 | 100 | 0 | 106 |
PI | |||||
10°C | 30 | 0 | 100 | 0 | 30 |
22°C | 35 | 0 | 100 | 0 | 35 |
30°C | 35 | 0 | 100 | 0 | 35 |
SQDG | |||||
10°C | 20 | 100 | 0 | 20 | 0 |
22°C | 23 | 100 | 0 | 23 | 0 |
30°C | 28 | 100 | 0 | 28 | 0 |
Total | |||||
10°C | 1000 | 460 | 540 | ||
22°C | 1000 | 445 | 555 | ||
30°C | 1000 | 428 | 572 |
Data were calculated based on results of Supplemental Table 1 and Figure 1 according to methods of Browse et al. (1986) and Shen et al. (2010). C16 represents the sum of 16:0, 16:l, 16:2, and 16:3; C18 represents the sum of 18:0, 18:1, 18:2, and 18:3.
An original input of 1000 mole of fatty acids biosynthesized in the chloroplast as acyl-ACP species.
Each lipid species was divided between the prokaryotic and eukaryotic pathways on the basis of content of C16 at the sn-2 position.