Table 2. Frequency of Three Modes of MT Generation in the Steady State (per μm2).
Moss Line | Frequency (×10−4/s μm2) (P Value) |
n | Total Area (µm2) | ||
---|---|---|---|---|---|
Cytoplasmic Nucleation | Branching Nucleation | Severing | |||
Control | 0.33 ± 0.19 | 0.21 ± 0.18 | 0.12 ± 0.19 | 21 Cells | 11,223 |
0.34 ± 0.16 | 0.20 ± 0.12 | 0.11 ± 0.10 | 6 Experiments | ||
γ-Tubulin RNAi | 0.24 ± 0.19 (0.12) | 0.15 ± 0.16 (0.24) | 0.10 ± 0.12 (0.76) | 21 Cells | 9,421 |
0.26 ± 0.19 (0.47) | 0.19 ± 0.19 (0.92) | 0.08 ± 0.04 (0.58) | 4 Experiments | ||
Augmin RNAi | 0.29 ± 0.19 (0.59) | 0.22 ± 0.16 (0.95) | 0.05 ± 0.09 (0.26) | 12 Cells | 4,583 |
0.30 ± 0.19 (0.76) | 0.24 ± 0.06 (0.64) | 0.04 ± 0.01 (0.29) | 3 Experiments |
Values are means ± sd. sd represents either cell-to-cell (top rows) or experiment-to-experiment (bottom rows) variability.