Table 1. General Features of Sequenced Diatom Genomes.
| F. solaris JPCC DA0580 | P. tricornutum | T. pseudonana | |
|---|---|---|---|
| Nuclear genome | |||
| Estimated genome size (Mb) | 24.9 (49.7)a | 27.4 | 32.4 |
| Chromosome | 42 (84)b | 33 | 24 |
| Predicted protein-coding genes | 11,448 (20,455)c | 10,402 | 11,776 |
| GC content (%) | 46 | 49 | 47 |
| Proportion of TE (%) | 16 | 6.4 | 7.3 |
| Chloroplast genome | |||
| Estimated genome size (kb) | 135 | 117 | 129 |
| Predicted protein-coding genes | 132 | 130 | 127 |
| GC content (%) | 32 | 33 | 31 |
| Mitochondria genome | |||
| Estimated genome size (kb) | >38.6d | 77.4 | 43.8 |
| Predicted protein-coding genes | 31 | 32 | 35 |
| GC content (%) | 28 | 35 | 30 |
The total nuclear genome assembly with gaps was 49.7 Mb, which is equal to the total read length. An allodiploid genome structure could cause an increase in genome size in comparison to other microalgae. Since the sizes of P. tricornutum and T. pseudonana were described as haploid, the genome size of F. solaris was determined as half the total length of the scaffolds (24.9 Mb) for better comparison.
Scaffold assembly resulted in the formation of 42 homoeologous chromosome pairs; thus, 84 types of chromosomes were confirmed.
Estimated number of homoeologous gene pairs: 9007.
Mitochondrial genome of F. solaris has repeat sequences that are unreadable by the high-throughput DNA sequencing method employed here. The mitochondrial genome did not include the repeat sequences.