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. 2015 Feb 12;8:21. doi: 10.1186/s13068-015-0208-0

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© Wang et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Phenotype of Δ rca-1 × Δ cre-1 progeny. F1-Δrca-1, rca-1 deletion progeny; F1-Δrca-1; Δcre-1, rca-1 and cre-1 double deletion progeny; M, protein marker. (A) Heat map of secreted proteins, cellulose hydrolysis activity (CMCase), exo-glucanase activity (pNPCase), and xylanase activity of progenies, Δrca-1, and Δcre-1 grown on corn straw for 4 and 7 days compared with wild type (WT). Relative activities versus WT are displayed by color codes. Statistical significance was performed using a two-tailed Student’s t-test. *P < 0.05; **P < 0.01. (B) SDS-PAGE of secreted proteins of progenies, Δrca-1, Δcre-1, and WT when grown on corn straw for 4 days. Values represent the means of at least three biological replicates.