Table 2. Thermal Stages of PCR.
| Stage | Temperature | Time | Cycle |
|---|---|---|---|
| Prog.1: Predenaturation | 94℃ | 6 | 1 |
| Prog.2: | 33 | ||
| Seg.1: Denaturation | 94℃ | 1 | |
| Seg.2: Annealing | 55℃ | 1 | |
| Seg.3: Extension | 70℃ | 1 | |
| Prog.3: Full Extension | 72℃ | 7 | 1 |
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