Fig. 1.

Disruption of AJs and basolateral components cause different effects on Yki activity. Confocal images of third instar imaginal discs of the indicated genotypes showing the effects of expression of different RNAi constructs under the control of the ptc-Gal4 driver. A–D show full stacks of the top view, and E–H show optical cross-sections. Yellow dashed lines indicate the position of the cross-sections. The ptc-Gal4 expression domains are marked by coexpression of GFP (green or gray in the ” panels). Discs were stained for β-galactosidase to reveal the expression of the Yki activity reporter ex-lacZ (ex-Z, red or gray in the ‘ panels). Knockdown of E-cad (B and F) or α-cat (C and G) caused cell-autonomous decrease and strong non-autonomous increase of ex-lacZ expression, whereas knockdown of scrib (D and H) caused mainly cell-autonomous up-regulation of ex-lacZ expression. Anterior is to the left for all discs.