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. 2015 Jan 26;112(6):1749–1754. doi: 10.1073/pnas.1416656112

Fig. 1.

Fig. 1.

Mdm2 and c-Src form a complex in vitro and in vivo. (A) Schematic of Mdm2 is shown (Top). His-tagged Mdm2 and GST-tagged SH3Src from a nickel pull-down of His-Mdm2, His-Mdm2 SH3 domain mutant 1, and His-Mdm2 SH3 domain mutant 2 were immunoblotted for GST and His antibodies. Input is GST-SH3Src. (B) Immunoprecipitations of endogenous Mdm2 from 293T (Left) or c-Src from MCF7 and U87 cell extracts (Center and Right) were probed with Src and Mdm2 antibodies. (C) Mdm2 and c-Src were detected by immunoblot of cytoplasmic and nuclear extracts from MCF7 cells. Detection of tubulin and PARP indicate extract purity.