Figure 1.

Bacterial growth, transcriptional levels of ROS-detoxification genes, intracellular ATP levels, and morphological changes in C. jejuni under microaerobic and aerobic conditions. (A) The growth kinetics of C. jejuni under microaerobic and aerobic culture conditions. The means and standard deviations of triplicate samples in a single experiment are indicated. The experiments were repeated three times. (B) Transcriptional levels of ahpC, katA, and sodB genes under microaerobic and aerobic culture conditions. C. jejuni strains harboring P_ahpC-lacZ, P_katA-lacZ, and P_sodB-lacZ were grown in MH medium microaerobically or aerobically. The results are representative of at least three independent experiments. Each experiment was performed with triplicate samples and all the repeated experiments exhibited similar results. (C) Intracellular ATP levels in C. jejuni under microaerobic and aerobic conditions. The ATP concentrations were normalized to the total protein concentrations. The experiment was performed in triplicate and was repeated three times. Error bars indicate the standard deviations. (D) Fluorescent microscopic images of C. jejuni with LIVE/DEAD BacLight™ staining under microaerobic and aerobic conditions. The scale bar shows 10 μm. “m/a” and “aero” indicate microaerobic and aerobic conditions, respectively.