Figure 2.

Effects of a perR mutation on the transcription of ahpC (A), sodB (B), and katA (C) under oxidative stress. β-Galactosidase assays of ahpC, sodB, and katA expression after exposure to 1 mM hydrogen peroxide (H₂O₂), 100 μM cumene hydroperoxide (CHP), and 100 μM menadione (MND). The promoter fusion assays were performed in both wild type and a perR mutant. After 8 h culture under microaerobic conditions, C. jejuni was exposed to oxidants for 2 h prior to an assay. The values represent the means and standard deviations of three independent experiments. Statistical analysis was performed with the Student's t-test using GraphPad Prism 6 (GraphPad Software Inc., USA). ^**, P < 0.01; ^***, P < 0.001; NS, not significant.