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. 2015 Feb 17;9:9. doi: 10.3389/fnana.2015.00009

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Copyright © 2015 Navarro, Alvarado, Navarrete, Giner, Obregon, Manzanares and Berbel.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Confocal immunolabeling in the somatosensory cortex of C and MMI pups. Confocal photomicrographs showing VGluT1-ir (green labeling; A,F,K), VGAT-ir (red labeling; B,G,L) and merged images (C,H,M) in the somatosensory cortex of C (A–E), MMI21 (F–J) and MMI10 (K–O) pups at P50. Asterisks (^*) point to supragranular VGluT1-ir labeling in MMI (F,K) pups. Perisomatic VGAT-ir boutons are indicated by arrows. Boxes in (B,G,L) show the location of the corresponding enlarged figures. Note the decreased density and smaller size of perisomatic VGAT-ir boutons (arrows) in layers II-III and V in MMI10 pups (compare N,O with D,E,I,J). Same scale for (A–C, F–G, K–M) and for (D,E,I,J,N,O).