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. 2015 Feb 17;9:9. doi: 10.3389/fnana.2015.00009

Figure 6.

Figure 6

Low magnification confocal images showing abnormal immunolabeling in MMI pups. Collages of confocal photomicrographs showing VGluT1-ir (green labeling; A,D,G), VGAT-ir (red labeling; B,E,H) and merged images (C,F,I) in the hippocampus of C (A–C), MMI21 (D–F) and MMI10 (G–I) pups at P50. Note the decreased VGluT1-ir in DG distal-inner molecular layer (arrowhead in A,D,G), in CA3 strata lucidum (arrow in A,D,G) and radiatum, and in CA1 stratum lacunosum-moleculare (double arrows in A,D,G) in MMI compared to C pups. These data show that the basic trisynaptic loop in MMI pups is abnormal. A narrow band of increased VGAT-ir boutons located at the border between the DG distal and proximal molecular layers of MMI pups is indicated (arrow heads in E,H). The borders of the principal cell layer, defined by VGAT immunolabeling) are more blurred in MMI pups compared to controls (arrows in B,E,H). Same scale for (A–I).