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. 2015 Feb 17;9:9. doi: 10.3389/fnana.2015.00009

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Copyright © 2015 Navarro, Alvarado, Navarrete, Giner, Obregon, Manzanares and Berbel.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Confocal immunolabeling in CA3 of C and MMI pups. Confocal photomicrographs showing VGluT1-ir (green labeling; A–C), VGAT-ir (red labeling; D–F) and merged images (G–I) in CA3 of C (A,D,G), MMI21 (B,E,H) and MMI10 (C,F,I) pups at P50. Note that the area occupied by VGluT1-ir mossy boutons in the strata oriens (o; arrows) and lucidum (l; arrowheads) of CA3 is less in MMI21 (B) and MMI10 (C) pups than in controls (A). The border between the strata lucidum (l) and radiatum (r) are marked in dashed lines in (B,C), owing to the low density of labeled boutons. p, Pyramidale; lm, lacunosum-moleculare. Same scale for (A–I).