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. 2015 Feb 12;16(1):1. doi: 10.1186/s12867-015-0029-5

Figure 2.

Figure 2

Overexpression of MKL1 induces MPC5 cell growth arrest. A) MPC5 cells were transiently transfected with a mouse MKL1 expression plasmid and cultured at 33°C. Expression of MKL1 protein was verified by western blotting. Actin was used to normalize MKL1 levels. B) At the indicated time points, cell growth was measured using the CCK-8 assay. *p < 0.05 and **p < 0.01 compared with the control (one-way ANOVA followed by Tukey’s HSD test). C) Cell proliferation was measured by immunofluorescence analysis of EdU incorporation. Scale bars, 25 μm. The percentage of proliferating cells was calculated as EdU-positive cells/Hoechst-stained cells × 100%. *p < 0.05 compared with the control (one-way ANOVA followed by Tukey’s HSD test). D) MPC5 cells were stably transfected with a mouse MKL1 expression plasmid and cultured at 33°C. Expression of MKL1 protein was verified by western blotting. Actin was used to normalize MKL1 levels. E) At the indicated time points, cell growth was measured using the CCK-8 assay. **p < 0.01 compared with the control (one-way ANOVA followed by Tukey’s HSD test). F) Cell proliferation was measured by immunofluorescence analysis of EdU incorporation. Scale bars, 25 μm. The percentage of proliferating cells was calculated as EdU-positive cells/Hoechst-stained cells × 100%. *p < 0.05 compared with the control (one-way ANOVA followed by Tukey’s HSD test).