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. 2015 Jan 7;34(4):491–501. doi: 10.15252/embj.201490177

Figure 3.

Figure 3

A heterologous amphipathic helix can functionally substitute for the N-terminus
  1. Helical wheel projection showing the amphipathic helix from hepatitis C virus protein NS4B. The hydrophobic face is indicated by an arrow. Colour scheme as in Fig2B.
  2. Growth of strains DWA350 (Δnoc ΔminCD) and DWA307 (Δnoc ΔminCD,Pxyl-HCVAH-NocNΔ10) on nutrient agar plates at 30 and 48°C in the absence and presence of 0.5% w/v xylose, as indicated.
  3. Cell morphology of strains DWA350 and DWA307 following growth in LB at 30°C (C and D) and at 42°C in LB + 0.5% w/v xylose (E and F). Arrowheads indicate minicells. Cell membranes were stained with FM5–95. Insets show the corresponding phase contrast images. Scale bar, 5 μm.
  4. Cellular localisation of HCVAH-NocNΔ10-YFP (G) in strain DWA193 (Δnoc,Pxyl-HCVAH-nocNΔ10-yfp) and overlay showing DAPI-stained DNA (H). The strain was grown at 30°C in CH medium. Inset shows the corresponding phase contrast image. Scale bar, 5 μm.