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. 2015 Jan 16;34(4):517–530. doi: 10.15252/embj.201489574

Figure 7.

Figure 7

p53 constrains NFATc1-dependent promotion of EMT phenotype and cancer stem cell-like properties through induction of miRNA-200c
  1. Expression analysis of indicated miRNAs upon adenoviral re-expression of p53 in KPΔNC tumor cells, detected by real-time PCR (P < 0.05).
  2. Protein expression of EMT marker genes after adenoviral re-expression of GFP-tagged p53 in KPΔNC tumor cells.
  3. Protein (C) and mRNA (D) expression levels of EMT and stemness marker genes upon miRNA-34a and miRNA-200c overexpression using specific mimics in KPNC tumor cells. Representative results from at least three independent experiments are shown (means ± SD;P < 0.05).
  4. Western blot analysis of EMT marker expression in KNC cells following depletion of miR-200c.
  5. ChIP experiments showing NFATc1 binding along with H3K27 acetylation mark at the selected Snai1 enhancer region as well as Sox2 and RNA polymerase II binding at Snai1 promoter after miR-200c overexpression in KPNC tumor cells. Means ± SD are shown from one out of three independent experiments.
  6. ChIP analysis in KPΔNC tumor cells after adenoviral GFP-tagged p53 overexpression. NFATc1 and H3K27ac binding are illustrated on Snai1 enhancer, and Sox2 and RNA polymerase II binding are demonstrated on Snai1 promoter. Means ± SD are shown from one out of three independent experiments.
  7. Quantification of KPΔNC spheres after adenoviral GFP-tagged p53 overexpression.
Source data are available online for this figure.