Skip to main content
. Author manuscript; available in PMC: 2015 Feb 17.
Published in final edited form as: Curr HIV Res. 2014;12(3):164–173. doi: 10.2174/1570162x12666140526122249

Figure 1. Higher level of HIV-1 replication in MDMs than monocytes, regardless of duration of monocyte adherence.

Figure 1

Monocytes were collected and adhered to tissue culture treated flasks for four hours or 24 hours prior to infection with HIV-1 Bal (10 ng p24/1×106 cells). Monocytes were also isolated by adherence for 7 days and maintained in the presence of GM-CSF (100 ng/ml) for seven days prior to HIV-1 infection. Seven days post-HIV-1 infection, HIV-1 p24 was measured by conventional ELISA (A) or intracellular flow cytometry for CD3, CD4, CD14, active β- catenin, and HIV-1 p24 (B). The median percentage of live, CD3-CD14+HIV-1 p24+ cells was determined (B). Data are based on at least three different donors. Asterisk denotes p<0.05 in comparison to first two columns.