Fig. 4.

Effect of insulin on absolute oxygen consumption. The basal mitochondrial respiratory rate (panel A), the oligomycin-sensitive rate (ATP turnover, panel B), the oligomycin-resistant rate (proton leak, panel C), and the FCCP-stimulated rate (maximum respiration, panel D) were expressed as total oxygen consumed per minute per XF24 well, and were calculated for human muscle cells incubated without (white bars) or with 100 nM insulin (black bars). The data shown are means ± SEM of 3–6 independent experiments. Because of density variation between myoblasts and myotubes, only internal comparisons ± insulin were tested statistically – the asterisks indicate significant differences (P < 0.05).