Fig. 7.

NIR difference spectra of oxidase species with and without CuA redox features.

Data from Fig. 1, Fig. 3A and 4B.

pH 8.75 & pH 7.4; 24.5 μM cytochrome c oxidase; other conditions as in Fig. 4A & B.

compound F minus oxidized (O) (VII − IX, Table 2); pH 8.75.

compound P minus oxidized (O) (VI − IX, Table 2); pH 8.75.

fully reduced minus oxidized (O) (I − IX, Table 2); pH 8.75.

mixed valence (cytochrome a^2 +, CuA⁺) formate complex minus oxidised formate complex (IV − VIII, Table 2); pH 7.4.