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. Author manuscript; available in PMC: 2015 Feb 17.
Published in final edited form as: DNA Repair (Amst). 2010 Apr 24;9(7):745–753. doi: 10.1016/j.dnarep.2010.03.010

Figure 1.

Figure 1

Timecourse showing siRNA mediated downregulation of ERCC1 and XPF protein in H1299 cells. Cells were transiently transfected twice at 24 h intervals, each time with smartpool siRNAs (100 nM) directed against ERCC1 (A), XPF (B) and both proteins (C, double knockdown). Control cells were left untransfected (UT), or mock (M) – and non-targeting (NT) siRNA (100 nM) – transfected. Proteins were extracted at the indicated time points of 72, 96 and 120 h post-transfection, and probed with XPF, ERCC1 and α-tubulin as a loading control. The percent of knockdown was quantified using the Alpha Innotech HD2 and is represented for all cell lines tested in Table 1. Blots from (A) and (B) were also probed for XPF or ERCC1 on individual knockdown of ERCC1 or XPF, respectively.