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. Author manuscript; available in PMC: 2015 Feb 17.
Published in final edited form as: DNA Repair (Amst). 2010 Apr 24;9(7):745–753. doi: 10.1016/j.dnarep.2010.03.010

Figure 2.

Figure 2

Transcript levels on siRNA mediated downregulation. (A) represents ERCC1 transcript levels. H1299 cells were mock (M) treated or non-targeting (NT)-, XPF-, ERCC1-, and XPF–ERCC1 (denoted as siX + siE) siRNA transfected, twice at 24 h intervals each and harvested at 48 h post-transfection. Total RNA was extracted from cells and analyzed using StaRT-PCR, as described in Section 2. Each PCR was run in triplicate. The transcript levels are represented as ERCC1 mRNA/106 ACTB mRNA. The values are represented as mean±SEM from triplicate PCRs. (B) represents XPF transcript levels. H1299 cells were mock (M) treated or non-targeting (NT)-, XPF-, ERCC1-, and XPF–ERCC1 (denoted as siX + siE) siRNA transfected, twice at 24 h intervals each and harvested at 48 h post-transfection. Total RNA was extracted from cells and analyzed using StaRT-PCR, as described. Each PCR was run in triplicate. The transcript levels are represented as XPF mRNA/106 ACTB mRNA. The values are represented as mean±SEM from triplicate PCRs.