Figure 6.

Colony survival in H1299 (A) and H1355 (B) cell lines. Non-targeting (NT, filled squares), siXPF (filled circles), siERCC1 (open circles) and siXPF–siERCC1 (filled triangles, denoted as siX + siE) siRNA transfected cells were treated with increasing doses of cisplatin for 2 h, and cell viability was determined by a clonogenic assay. The mock treated cells were left off of the graph for clarity reasons, but resulted in cytotoxicity nearly identical to non-targeting siRNA (data not shown). Calculated cisplatin IC₅₀s for transfected cells are summarized in Table 1. Table 1 also summarizes the IC₅₀s for control and knockdown cells for all other cell lines tested. Values are represented as mean±SEM from three independent experiments.