Table 1.

Comparison of γH2AX analysis methods.

Analysis method	Readout	Sensitivity	Time	Sample prep cost	Instrument cost	# cells analyzed
Image cytometry	Per cell average intensity	2+	1+	2+	3+	100–20,000
Western blot	Specimen average	1+	2+	1+	1+	100,000–1,000,000
Immunofluorescence microscopy	Per cell foci	3+	3+	2+	2+ – 3+	10–100
Flow cytometry	Per cell average intensity	2+	2+	3+	3+ – 3+	5000–10,000
Imagestream flow cytometry	Per cell foci	3+	3+	3+	4+	5000–10,000

Common methods utilized for analysis of γH2AX are compared across a range of use parameters. An arbitrary scale of 1+ (low) to 4+ (high) is used.