Skip to main content
. Author manuscript; available in PMC: 2015 Feb 17.
Published in final edited form as: Integr Biol (Camb). 2014 Jan;6(1):44–52. doi: 10.1039/c3ib40140e

Fig 6. Inhibition of c-met is associated with lower level of epithelial marker expression.

Fig 6

(A) Hepatocytes were cultured onto micropatterned surfaces containing alternating HGF and TGF-β spots in absence (i) and presence (ii) of HGF receptor (c-met) inhibitor, SU11274. Spheroid formation on HGF was not observed in hepatocytes cultured for five days in presence of c-met inhibitor. The center-to-center distance between spots was 1.5 mm and spot size was 300 μm diameter. (B) Immunostaining images showing the lower expression of epithelia cell marker (E-cadherin) and higher expression of mesenchymal marker (N-cadherin) in cells cultured for four days on both HGF and TGF-β1 spots in presence of SU11274. Hepatocytes cultured on micropatterened surfaces containing alternating spots of HGF and TGF-β1 spots in absence of c-met inhibitor were used as control. The center-to-center distance between spots was 0.7 mm and 1.5 mm. (C, D) The fluorescence intensity of E-cadherin and N-cadherin was quantified using imageJ software and normalized with their DAPI intensity. Data are mean ± SD, n=3. Scale bar: 100 μm.