Skip to main content
. 2015 Feb 17;10(2):e0118063. doi: 10.1371/journal.pone.0118063

Fig 2. Growth of wild type B. burgdorferi 297 (solid circles), Δrel Bbu (open circles), or Δrel Bbu complemented with pKFSS1-Δrel Bbu (solid triangles) in BSK-Lite containing either glucose or glycerol as principal carbon sources.

Fig 2

Insets show enlargement of cell concentrations for clarity. Glucose-containing medium, 34°C (A). Differences between stationary phase cell concentrations of wild type and Δrel Bbu mutant are significant as are differences in stationary phase concentrations between the Δrel Bbu mutant and its complemented derivative (P < 0.001, one-way ANOVA, Bonferroni post-test). Glycerol-containing medium, 34°C (B). Differences between stationary phase cell concentrations of wild type and Δrel Bbu mutant are significant (P < 0.001, one-way ANOVA, Bonferroni post-test). Differences in stationary phase concentrations between the Δrel Bbu mutant and its complemented derivative are not significant (P > 0.05, one-way ANOVA, Bonferroni post-test). Glucose-containing medium, 25°C (C). Differences between stationary phase cell concentrations of wild type and Δrel Bbu mutant are significant as are differences in stationary phase concentrations between the Δrel Bbu mutant and its complemented derivative (P < 0.001, one-way ANOVA, Bonferroni post-test). Glycerol-containing medium, 25°C (D). Differences between stationary phase cell concentrations of wild type and Δrel Bbu mutant are significant (P < 0.001, one-way ANOVA, Bonferroni post-test) as are differences in stationary phase concentrations between the Δrel Bbu mutant and its complemented derivative (P < 0.05, one-way ANOVA, Bonferroni post-test).