Table 1. Modulated selected genes with annotated function in B. burgdorferi 297 Δrel Bbu during growth in vitro at 34°C a .
| Exponential phase | Stationary phase | ||||
|---|---|---|---|---|---|
| Gene | Description | Mean expression (log2 [ΔrelBbu/WT]) | P | Mean expression (log2 [ΔrelBbu/WT]) | P |
| Transcriptional regulators | |||||
| BB0168 | dnaK suppressor (dksA) | 1.31 | 0.002 | 3.26 | <0.001 |
| BB0419 | response regulatory protein (rrp-1) | 1.42 | 0.001 | ||
| BB0420 | sensory transduction histidine kinase/response regulator (hk1) | 2.65 | 0.001 | 2.39 | <0.001 |
| BB0712 | RNA polymerase σ70 factor (rpoD) | 1.05 | 0.013 | 2.19 | <0.001 |
| DNA synthesis/repair | |||||
| BB0022 | Holliday junction DNA helicase (ruvB) | 2.58 | <0.001 | ||
| BB0344 | DNA helicase (uvrD) | 2.79 | 0.006 | ||
| BB0438 | DNA polymerase III, β subunit (dnaN) | 2.41 | 0.006 | 1.56 | <0.001 |
| BB0552 | DNA ligase (lig) | 2.64 | 0.010 | ||
| BB0579 | DNA polymerase III, α subunit (dnaE) | 4.95 | 0.001 | ||
| BB0710 | DNA primase (dnaG), authentic frameshift | 1.26 | <0.001 | 1.33 | <0.001 |
| BB0836 | excinuclease ABC, B subunit (uvrB) | 2.35 | <0.001 | ||
| BB0837 | excinuclease ABC, A subunit (uvrA) | 1.04 | 0.003 | ||
| Cell division | |||||
| BB0177 | glucose inhibited division protein B (gidB) | 3.81 | 0.007 | ||
| BB0178 | glucose inhibited division protein A (gidA) | 3.07 | 0.005 | ||
| BB0302 | cell division protein (ftsW) | 2.96 | 0.001 | ||
| BB0781 | GTP-binding protein (obg) | 1.03 | 0.004 | 1.95 | <0.001 |
| BB0789 | cell division protein (ftsH) | 1.01 | <0.001 | ||
| Protein synthesis | |||||
| BB0229 | ribosomal protein L31 (rpmE) | 1.54 | <0.001 | ||
| BB0251 | leucyl-tRNA synthetase (leuS) | 3.41 | <0.001 | ||
| BB0514 | phenylalanyl-tRNA synthetase, β subunit (pheT) | 1.63 | 0.009 | ||
| BB0615 | ribosomal protein S4 (rpsD) | 2.87 | <0.001 | ||
| BB0691 | translation elongation factor G (fus-2) | 0.96 | 0.001 | ||
| BB0778 | ribosomal protein L21 (rplU) | 1.51 | 0.002 | ||
| BB0780 | ribosomal protein L27 (rpmA) | 1.03 | <0.001 | ||
| Motility/chemotaxis | |||||
| BB0147 | flagellar filament 41 kDa core protein (flaB) | -1.31 | 0.001 | ||
| BB0181 | flagellar hook-associated protein (flgK) | -1.14 | 0.010 | ||
| BB0271 | flagellar biosynthesis protein (flhA) | 1.95 | 0.002 | ||
| BB0578 | methyl-accepting chemotaxis protein (mcp-1) | 3.06 | 0.012 | ||
| BB0668 | flagellar filament outer layer protein (flaA) | -1.13 | <0.001 | ||
| BB0670 | purine-binding chemotaxis protein (cheW-3) | 2.03 | 0.011 | ||
| BB0775 | flagellar hook-basal body complex protein (flhO) | 1.03 | <0.001 | ||
| Cell envelope | |||||
| BB0382d | basic membrane protein B (bmpB) | -1.02 | 0.012 | -1.59 | <0.001 |
| BB0383 | basic membrane protein A (bmpA) | -1.15 | 0.016 | ||
| BB0385 | basic membrane protein D (bmpD) | -2.93 | 0.007 | ||
| BBA15 | outer surface protein A (ospA) | -1.81 | 0.001 | ||
| BBA16 | outer surface protein B (ospB) | -1.54 | 0.002 | ||
| BBA60 | surface lipoprotein P27 | -5.07 | <0.001 | ||
| BBA74 | membrane-associated periplasmic protein | -2.03 | <0.001 | ||
| BBB07 | α3β1 integrin-binding protein | 1.42 | <0.001 | ||
| BBJ41 | antigen P35, putative | -8.62 | <0.001 | ||
| BBM23 b | holin (blyA) | 1.74 | <0.001 | 1.43 | <0.001 |
| BBN24 c | holin (blyB) | 2.99 | <0.001 | 1.17 | <0.001 |
| Central metabolism/carbon source transporters | |||||
| BB0240 | glycerol uptake facilitator (glpF) | -4.54 | <0.001 | -4.76 | <0.001 |
| BB0241 | glycerol kinase (glpK) | -8.27 | <0.001 | -5.47 | <0.001 |
| BB0243 | glycerol-3-phosphate dehydrogenase (glpD) | -6.52 | <0.001 | -3.79 | <0.001 |
| BB0328 | oligopeptide ABC transporter, periplasmic oligopeptide-binding protein (oppA-1) | -1.16 | 0.001 | -2.65 | 0.002 |
| BB0329 | oligopeptide ABC transporter, periplasmic oligopeptide-binding protein (oppA-2) | -1.33 | <0.001 | ||
| BB0330 | oligopeptide ABC transporter, periplasmic oligopeptide-binding protein (oppA-3) | -4.07 | 0.015 | ||
| BB0334 | oligopeptide ABC transporter, ATP-binding protein (oppD) | -3.25 | 0.006 | -1.21 | 0.002 |
| BB0335 | oligopeptide ABC transporter, ATP-binding protein (oppF) | -1.51 | <0.001 | ||
| BBB04 | chitobiose transporter protein (chbC) | 1.83 | 0.004 | 2.11 | <0.001 |
| BBB05 | chitobiose transporter protein (chbA) | 4.05 | 0.013 | 5.07 | <0.001 |
| BBB06 | chitobiose transporter protein (chbB) | 5.02 | <0.001 | ||
| BB0683 | 3-hydroxy-3-methylglutaryl-CoA synthase (hmgs) | 1.23 | 0.010 | 1.65 | <0.001 |
| BB0685 | 3-hydroxy-3-methylglutaryl-CoA reductase (mvaA) | 1.36 | <0.001 | 4.39 | <0.001 |
a. Transcriptional analysis from microarrays (regular font) or RT-PCR (boldface). Where data from RT-PCR is shown, microarrays showed no significant difference in gene expression between B. burgdorferi 297 Δrel Bbu and wild type.
b. Expression values for blyA orthologs BBM23, BBP23, BBR23 that showed increased expression in stationary phase and BBN23, BBR23 and BBS23 that showed increased expression in exponential phase were considered as a single transcript because they are 100% identical in sequence.
c. Expression values for blyB orthologs BBN24, BBR24, BBS24 that showed increased expression in stationary phase and BBN24, BBR24, and BBS23 that showed increased expression in exponential phase were considered as a single transcript because they are 100% identical in sequence.