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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Toxicol Appl Pharmacol. 2015 Jan 10;283(2):99–108. doi: 10.1016/j.taap.2014.12.017

Figure 1. Thymus cellularity and efficiency of thymocyte conversion in adult male C57BL/6 or Notch1ICN-TG mice after administration of TCDD or vehicle control.

Figure 1

Wild type C57BL/6 or Notch1ICN-TG mice were administered 10μg/kg TCDD, 30μg/kg TCDD or vehicle control via oral gavage at 4 weeks of age. Ten days later, mice were euthanized, thymuses removed, single cell suspensions made, and cells stained with fluorescent-conjugated antibodies directed against CD4 and CD8. Cells were acquired on a BD FACS Aria III flow cytometer and data analyzed in FlowJo. A) Thymus weight ± SEM; B) Thymocyte cellularity ± SEM; C–F) Absolute cell number (mean ± SEM) in each thymocyte cell population; G–H) Efficiency of SP (CD4+ or CD8+) generation, calculated by dividing the number of CD4+ or CD8+ thymocytes in by the number of CD4+CD8+ precursors; Vehicle mice are represented with white bars, 10μg/kg TCDD mice with black bars, and 30μg/kg TCDD-exposed mice with gray bars. A single * indicates p ≤0.05; ** indicates p ≤0.01; *** indicates p ≤0.001. All compared to vehicle control within genotype unless otherwise indicated by bracket. (n=at least 4 B6 mice in each group; n=at least 8 Notch1ICN-TG mice in each group)