Figure 4. Mitochondrial hOggl co-precipitates with mitochondrial aconitase.

Immunoprecipitation (IP) using a hOggl antibody was performed on mitochondria proteins obtained from control and mt-hOggl overexpressing A549 cells in the absence or presence of asbestos (25 µg/cm²) for 24 h as described in the Methods. Mitochondrial aconitase and loading controls (IgG and hOggl) were assessed by Western blotting (WB). The immunoprecipitation blots are representative of three independent experiments.